The utility model discloses a test machine station for a trolley mouse, including a height adjustment station, a mouse bearing mechanism, a rolling generation mechanism, and a data processor. The height adjustment station is used to connect and to adjust the mouse bearing mechanism at certain inclined angle. The mouse bearing mechanism is made to bear and fix the trolley mouse to be tested. While the trolley mouse bearing mechanism is adjusted to an inclined condition, the bearing trolley wheel is also inclined with such angle. The rolling generation mechanism with at least one roller is controlled by the data processor. The roller touches with the trolley of the trolley mouse. The trolley is driven and rolls upon meeting the trolley during the rolling. The data processor, based on at least one test item, generates the controlling signals for the testing item so as to control the rolling generation mechanism, receives the trolley signals from the trolley mouse, process the trolley signals, and export the test results to the trolley mouse.